PE/Cy5.5® Anti-CD45 antibody [HI30] (ab167004) (2024)

Overview

• Product name

  PE/Cy5.5® Anti-CD45 antibody [HI30]
  See all CD45 primary antibodies

• Description

  PE/Cy5.5® Mouse monoclonal [HI30] to CD45

• Host species

  Mouse

• Conjugation

  PE/Cy5.5®. Ex: 496nm, Em: 690nm

• Tested applications

  Suitable for: Flow Cytmore details

• Species reactivity

  Reacts with: Human
  

• Immunogen

  Tissue, cells or virus corresponding to Human CD45. Human Peripheral Blood Leukocytes

• Positive control

  • Human peripheral blood cells

• General notes

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

• Form

  Liquid

• Storage instructions

  Shipped at 4°C. Store at +4°C.

• Storage buffer

  pH: 7.20
  Preservative: 0.09% Sodium azide
  Constituent: 99% PBS

• Concentration information loading...

• Purity

  Protein G purified

• Clonality

  Monoclonal

• Clone number

  HI30

• Isotype

  IgG1

• Light chain type

  kappa

• Research areas

  • Immunology
  • Adaptive Immunity
  • B Cells
  • CD

  • Immunology
  • Adaptive Immunity
  • T Cells
  • CD

  • Immunology
  • Cell Type Markers
  • CD
  • Non-lineage

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Phosphatases

  • Neuroscience
  • Cell Type Marker
  • Glia marker
  • Microglia marker

  • Stem Cells
  • Hematopoietic Progenitors
  • Surface Molecules

  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • B Lymphocytic Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • T Lymphocytic Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • NK Cell Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Dendritic Cell Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Monocytic Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Neutrophil Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Mast Cell Lineage

  • Immunology
  • Adaptive Immunity
  • Regulatory T Cells

  • Cardiovascular
  • Angiogenesis
  • Endothelial Cell Markers

  • Neuroscience
  • Development

Associated products

• Alternative Versions

  • Anti-CD45 antibody [HI30] (ab123522)
  • PE/Cy7® Anti-CD45 antibody [HI30] (ab155340)
  • PE/Cy5® Anti-CD45 antibody [HI30], prediluted (ab155385)
  • APC Anti-CD45 antibody [HI30] (ab210270)
  • FITC Anti-CD45 antibody [HI30] (ab210272)
  • PE Anti-CD45 antibody [HI30] (ab210273)
  • redFluor™ 710 Anti-CD45 antibody [HI30] (ab242257)
  • violetFluor™ 450 Anti-CD45 antibody [HI30] (ab253085)

• Recombinant Protein

  • Recombinant human CD45 protein (Fc Chimera Active) (ab214825)

Applications

Target

• Function

  Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

• Involvement in disease

  Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.

• Sequence similarities

  Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
  Contains 2 fibronectin type-III domains.
  Contains 2 tyrosine-protein phosphatase domains.

• Domain

  The first PTPase domain interacts with SKAP1.

• Post-translational
  modifications

  Heavily N- and O-glycosylated.

• Cellular localization

  Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.

• Information by UniProt

• Database links

  • Entrez Gene: 5788 Human
  • Omim: 151460 Human
  • SwissProt: P08575 Human
  • Unigene: 654514 Human

• Alternative names

  • B220 antibody
  • CD 45 antibody
  • CD45 antibody

  see all

Images

Protocols

Datasheets and documents

References (0)